Differential macrophage polarisation during parasitic infections in common carp (Cyprinus carpio L.)

被引：42

作者：

Joerink, Maaike ^{[1
]}

Forlenza, Maria ^{[1
]}

Ribeiro, Carla M. S. ^{[1
]}

de Vries, Beitske J. ^{[1
]}

Savelkoul, Huub F. J. ^{[1
]}

Wiegertjes, Geert F. ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Wageningen Inst Anim Sci, Dept Anim Sci, Cell Biol & Immunol Grp, NL-6700 AH Wageningen, Netherlands

来源：

FISH & SHELLFISH IMMUNOLOGY | 2006年 / 21卷 / 05期

关键词：

macrophage; alternative activation; classical activation; iNOS; arginase; gene expression; re-stimulation;

D O I：

10.1016/j.fsi.2006.03.006

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 [水产];

摘要：

in many parasitic infections both classically activated macrophages (caMF) and alternatively activated rnacrophages (aaMF) play a pivotal role. To investigate if both types of macrophages also play an important role during parasitic infections in fish, we infected carp with either Trypanoplasma borreli or Trypanosoma carassii and determined the activation state of the head kidney leukocytes (HKL). Nitrite production was used as read-out for caMF and arginase activity as read-out for aaMF. Basal nitrite production and arginase activity of HKL were moderately different between the two infections. Differences were observed, however, after ex vivo re-stimulation of HKL. Re-stimulation with LPS and T. borreli lysates increased nitrite production by HKL of T. borreli-infected fish. Re-stimulation with cAMP increased arginase activity in HKL of T. carassii-infected fish. Our results indicate that T. borreli-infected carp are more prone to increase nitrite production by caMF while T. carassii-infected fish are more prone to increase arginase activity by aaMF. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：561 / 571

页数：11

共 34 条

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