Isocitrate lyase localisation in Saccharomyces cerevisiae cells

被引：17

作者：

Chaves, RS ^{[1
]}

Herrero, P ^{[1
]}

Ordiz, I ^{[1
]}

delBrio, MA ^{[1
]}

Moreno, F ^{[1
]}

机构：

[1] UNIV OVIEDO,INST BIOTECNOL ASTURIAS,DEPT BIOQUIM & BIOL MOL,E-33006 OVIEDO,SPAIN

来源：

GENE | 1997年 / 198卷 / 1-2期

关键词：

ICL1; glyoxylate; gene expression; GFP;

D O I：

10.1016/S0378-1119(97)00311-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

The isocitrate lyase from Saccharomyces cerevisiae was only located in the cell cytoplasm. This protein was found not to be associated with cell organelles, even under growth conditions that induce peroxisome proliferation. This conclusion is supported by experiments carried out by damaging the protoplast plasma membrane with DEAE-dextran, by differential centrifugation of osmotically lysed protoplast and by using the green fluorescent protein (GFP) of Aequorea victoria as a reporter fusion tag to localise the subcellular compartment to which isocitrate lyase is targeted. (C) 1997 Elsevier Science B.V.

引用

页码：165 / 169

页数：5

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