Simplified method for estimation of microbial activity in compost by ATP analysis

被引：41

作者：

Horiuchi, JI

Ebie, K

Tada, K

Kobayashi, M

Kanno, T

机构：

[1] Kitami Inst Technol, Dept Chem Syst Engn, Kitami, Hokkaido 0908507, Japan

[2] Kitami Inst Technol, Dept Civil Engn, Kitami, Hokkaido 0908507, Japan

[3] Kitami Inst Technol, Int Students Ctr, Kitami, Hokkaido 0908507, Japan

来源：

BIORESOURCE TECHNOLOGY | 2003年 / 86卷 / 01期

关键词：

compost; ATP; cell mass; monitoring; recombinant luciferase;

D O I：

10.1016/S0960-8524(02)00108-6

中图分类号：

S2 [农业工程];

学科分类号：

0828 [农业工程];

摘要：

A simplified method using ATP analysis was proposed to estimate the time course of microbial activity during composting. A compost sample was suspended in distilled water and its filtrate was used to estimate the ATP content in the compost by the luciferine-luciferase fluorescence reaction. The method permitted the rapid and simple determination of ATP in the compost and was successfully applied to monitor the time course of the microbial activity in the laboratory-scale composting process. The analytical simplicity in this method greatly improved the field-applicability of the ATP analysis for the composting process monitoring. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：95 / 98

页数：4

共 10 条

[1]

ADENYLATE ENERGY CHARGE IN SACCHAROMYCES-CEREVISIAE DURING STARVATION [J].