HPLC enantiomeric resolution of novel tetralone derivatives on cellulose and amylose based chiral stationary phases under normal phase mode

A comparison or the enantiomeric resolution of several tetralone derivatives, which are 17alpha-hydroxylase/17,20-lyase (P450 17alpha) inhibitors has been achieved on different polysaccharides based chiral stationary phases, namely, Chiralcel OB, Chiralcel OD, Chiralpak AD, Chiralpak AS, and Chiralcel OE The mobile phase used was hexane: ethanol: methanol (90: 7: 3 v/v/v) containing 0.5% triethylamine. Most of the compounds were completely separated on the polysaccharide based chiral stationary phases used in this study, with the exception of Chiralcel CA. The chromatographic parameters: the capacity factor (k), separation factor (alpha), and resolution factor (Rs) were calculated. The chiral recognition mechanisms between these analytes and chiral selectors are discussed.