ORF3 protein of hepatitis E virus is not required for replication, virion assembly, or infection of hepatoma cells in vitro

被引：92

作者：

Emerson, Suzanne U. ^{[1
]}

Nguyen, Hanh ^{[1
]}

Torian, Udana ^{[1
]}

Purcell, Robert H. ^{[1
]}

机构：

[1] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF VIROLOGY | 2006年 / 80卷 / 21期

关键词：

D O I：

10.1128/JVI.00892-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A subclone of Huh-7 cells that could be relatively efficiently transfected and infected with hepatitis E virus was identified. Following transfection, infectious virus was produced but remained predominantly cell associated. Intracellular virus, recovered by lysis of transfected cells, infected naive cells. This in vitro-produced virus appeared to be antigenically identical to virus isolated from clinical samples. Lysates from cells transfected with mutant viral genomes unable to synthesize ORF3 protein contained infectious virions that were similar in number, thermostability, and sedimentation characteristics to those in lysates transfected with wild-type viral genomes. Therefore, in contrast to its requirement in vivo, ORF3 protein is not required for infection of Huh-7 cells or production of infectious virus in vitro.

引用

页码：10457 / 10464

页数：8

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