Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase

被引：51

作者：

Wang, HL

Zou, HF ^{[1
]}

Ni, JY

Kong, L

Gao, S

Guo, BC

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China

[2] Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 870卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Artemisia capillaris; scoparone; capillarisin;

D O I：

10.1016/S0021-9673(99)01062-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the screening and analysis of biologically active compounds in traditional Chinese medicine is proposed. Affinity chromatography using a human serum albumin (HSA) stationary phase was applied to separate and analyze the bioactive compounds from Artemisia capillaris Thunb. Five major peaks and several minor peaks were resolved based on their affinity to HSA, two of them were identified as scoparone (SCO, 6,7-dimethoxycoumarin) and capillarisin (CAP). CAP shows a much higher affinity to HSA than SCO. The effects of acetonitrile concentration, eluent pH, phosphate concentration and temperature on the retention behaviors of several major active components were also investigated, and it was found that hydrophobicity and eluent pH play major roles in changing retention values. The results demonstrate that the affinity chromatography with a HSA stationary phase is an effective way for analyzing and screening biologically active compounds in traditional Chinese medicine. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：501 / 510

页数：10

共 21 条

[1]

Anetai Masaki, 1994, Natural Medicines, V48, P244

[2] USE OF A HUMAN SERUM ALBUMIN-BASED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY CHIRAL STATIONARY PHASE FOR THE INVESTIGATION OF PROTEIN-BINDING - DETECTION OF THE ALLOSTERIC INTERACTION BETWEEN WARFARIN AND BENZODIAZEPINE BINDING-SITES [J].