Large Scale Localization of Protein Phosphorylation by Use of Electron Capture Dissociation Mass Spectrometry

被引：75

作者：

Sweet, Steve M. M. ^{[1
,2
]}

Bailey, Christopher M. ^{[1
,2
]}

Cunningham, Debbie L. ^{[1
,2
]}

Heath, John K. ^{[1
,2
]}

Cooper, Helen J. ^{[1
,2
]}

机构：

[1] Univ Birmingham, Coll Life & Environm Sci, Sch Biosci, Birmingham B15 2TT, W Midlands, England

[2] Univ Birmingham, Coll Life & Environm Sci, Canc Res UK Growth Factor Grp, Birmingham B15 2TT, W Midlands, England

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2009年 / 8卷 / 05期

基金：

英国惠康基金;

关键词：

COLLISION-INDUCED DISSOCIATION; TYROSINE PHOSPHORYLATION; SACCHAROMYCES-CEREVISIAE; SITE LOCALIZATION; ION-TRAP; IN-VIVO; IDENTIFICATION; PEPTIDES; SEQUENCE; MS/MS;

D O I：

10.1074/mcp.M800451-MCP200

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID for phosphopeptide identification and site localization. Linear ion trap CID was shown to be most efficient for phosphopeptide identification, whereas FT-ICR ECD was superior for localization of sites of phosphorylation. The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment. Molecular & Cellular Proteomics 8: 904-912, 2009.

引用

页码：904 / 912

页数：9

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