Purification and initial crystallization studies of a DnaB intein from Synechocystis sp PCC 6803

被引：3

作者：

Chen, XH

Xu, MQ

Ding, Y

Ferrandon, S

Rao, ZH ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Biol Sci & Technol, Struct Biol Lab, Beijing 100084, Peoples R China

[2] Tsing Hua Univ, MOE Lab Prot Source, Beijing 100084, Peoples R China

[3] New England Biolabs Inc, Beverly, MA 01915 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444902006583

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A 154-residue mini-intein from the dnaB gene of Synechocystis sp. PCC 6803 (Ssp DnaB intein) has been purified and crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 58.2, c = 70.3 Angstrom. It has one molecule per asymmetric unit and diffracts to beyond 2.0 Angstrom under cryoconditions (100 K) using a rotating copper anode X-ray generator.

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页码：1201 / 1203

页数：3

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