Functional interaction between SHPTP1 and the Lyn tyrosine kinase in the apoptotic response to DNA damage

被引：52

作者：

Yoshida, K ^{[1
]}

Kharbanda, S ^{[1
]}

Kufe, D ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 49期

关键词：

D O I：

10.1074/jbc.274.49.34663

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Lyn protein-tyrosine kinase is activated in the cellular response to DNA-damaging agents. Here we demonstrate that Lyn associates constitutively with the SHPTP1 protein-tyrosine phosphatase. The SH3 domain of Lyn interacts directly with SHPTP1. The results show that Lyn phosphorylates SHPTP1 at the C-terminal Tyr-564 site. Lyn-mediated phosphorylation of SHPTP1 stimulates SHPTP1 tyrosine phosphatase activity. We also demonstrate that treatment of cells with 1-beta-D-arabinofuranosylcytosine and other genotoxic agents induces Lyn-dependent phosphorylation and activation of SHPTP1. The significance of the Lyn-SHPTP1 interaction is supported by the demonstration that activation of Lyn contributes in part to the apoptotic response to ara-C treatment and that SHPTP1 attenuates this response. These findings support a functional interaction between Lyn and SHPTP1 in the response to DNA damage.

引用

页码：34663 / 34668

页数：6

共 27 条

[1] Ataxia telangiectasia mutant protein activates c-Abl tyrosine kinase in response to ionizing radiation [J].

Baskaran, R ;

Wood, LD ;

Whitaker, LL ;

Canman, CE ;

Morgan, SE ;

Xu, Y ;

Barlow, C ;

Baltimore, D ;

WynshawBoris, A ;

Kastan, MB ;

Wang, JYJ .

NATURE, 1997, 387 (6632) :516-519

[2] C-ELEGANS CELL-SIGNALING GENE SEM-5 ENCODES A PROTEIN WITH SH2 AND SH3 DOMAINS [J].

CLARK, SG ;

STERN, MJ ;

HORVITZ, HR .

NATURE, 1992, 356 (6367) :340-344

[3] Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis [J].

Datta, R ;

Kojima, H ;

Banach, D ;

Bump, NJ ;

Talanian, RV ;

Alnemri, ES ;

Weichselbaum, RR ;

Wong, WW ;

Kufe, DW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (03) :1965-1969

[4]

GOGA A, 1995, ONCOGENE, V11, P791

[5] The stress response to ionizing radiation involves c-Abl-dependent phosphorylation of SHPTP1 [J].

Kharbanda, S ;

Bharti, A ;

Pei, D ;

Wang, J ;

Pandey, P ;

Ren, R ;

Weichselbaum, R ;

Walsh, CT ;

Kufe, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (14) :6898-6901

[6] IONIZING-RADIATION STIMULATES A GRB2-MEDIATED ASSOCIATION OF THE STRESS-ACTIVATED PROTEIN-KINASE WITH PHOSPHATIDYLINOSITOL 3-KINASE [J].

KHARBANDA, S ;

SALEEM, A ;

SHAFMAN, T ;

EMOTO, Y ;

TANEJA, N ;

RUBIN, E ;

WEICHSELBAUM, R ;

WOODGETT, J ;

AVRUCH, J ;

KYRIAKIS, J ;

KUFE, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (32) :18871-18874

[7] Functional interaction between DNA-PK and c-Abl in response to DNA damage [J].

Kharbanda, S ;

Pandey, P ;

Jin, SF ;

Inoue, S ;

Bharti, A ;

Yuan, ZM ;

Weichselbaum, R ;

Weaver, D ;

Kufe, D .

NATURE, 1997, 386 (6626) :732-735

[8] ACTIVATION OF THE C-ABL TYROSINE KINASE IN THE STRESS-RESPONSE TO DNA-DAMAGING AGENTS [J].

KHARBANDA, S ;

REN, RB ;

PANDEY, P ;

SHAFMAN, TD ;

FELLER, SM ;

WEICHSELBAUM, RR ;

KUFE, DW .

NATURE, 1995, 376 (6543) :785-788

[9] c-Abl activation regulates induction of the SEK1 stress-activated protein kinase pathway in the cellular response to 1-beta-D-arabinofuranosylcytosine [J].

Kharbanda, S ;

Pandey, P ;

Ren, R ;

Mayer, B ;

Zon, L ;

Kufe, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (51) :30278-30281

[10]

KHARBANDA S, 1994, ONCOGENE, V9, P3005

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