Efficient bioluminescence of bisdeoxycoelenterazine with the luciferase of a deep-sea shrimp Oplophorus

被引：28

作者：

Nakamura, H

Wu, C

Murai, A

Inouye, S

Shimomura, O

机构：

[1] CHISSO CORP,YOKOHAMA RES CTR,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

[2] MARINE BIOL LAB,WOODS HOLE,MA 02543

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 36期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0040-4039(97)01438-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The luminescence of the bisdeoxy analogue of coelenterazine (1b : X=Y=H) catalyzed by Oplophorus luciferase was highly efficient (0.19 quanta/molecule), almost comparable to the luminescence of unmodified coelenterazine (1A : X=Y=OH), whereas the luminescence of Ib was very inefficient when catalyzed by apoaequorin or Renilla luciferase and when the aequorin regenerated with 1b was luminesced with Ca2+. (C) 1997 Elsevier Science Ltd.

引用

页码：6405 / 6406

页数：2

共 11 条

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