Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

被引：47

作者：

Gorr, Ingo H.

Reis, Alexandra

Boos, Dominik

Wuehr, Martin

Madgwick, Suzanne

Jones, Keith T.

Stemmann, Olaf

机构：

[1] Univ Newcastle Upon Tyne, Sch Med, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

[2] Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany

来源：

NATURE CELL BIOLOGY | 2006年 / 8卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/ncb1467

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polarbody extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

引用

页码：1035 / U120

页数：5

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