Abundant Tax protein expression in CD4+T cells infected with human T-cell lymphotropic virus type I (HTLV-I) is prevented by cytotoxic T lymphocytes

被引:231
作者
Hanon, E
Hall, S
Taylor, GP
Saito, M
Davis, R
Tanaka, Y
Usuku, K
Osame, M
Weber, JN
Bangham, CRM
机构
[1] Univ London Imperial Coll Sci Technol & Med, Sch Med, Dept Immunol & Genitourinary Med & Communicable D, London W2 1PG, England
[2] Univ Ryukyus, Fac Med, Okinawa Asia Res Ctr Med Sci, Dept Infect Dis & Immunol, Nishihara, Okinawa 90301, Japan
[3] Kagoshima Univ, Fac Med, Dept Med Informat, Kagoshima 890, Japan
[4] Kagoshima Univ, Fac Med, Dept Internal Med 3, Kagoshima 890, Japan
关键词
D O I
10.1182/blood.V95.4.1386.004k22_1386_1392
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The role of the cellular immune response in human T-cell leukemia Virus type I (HTLV-I) infection is not fully understood. A persistently activated cytotoxic T lymphocyte (CTL) response to HTLV-I is found in the majority of infected individuals. However, it remains unclear whether this CTL response is protective or causes tissue damage. In addition, several observations paradoxically suggest that HTLV-I is transcriptionally silent in most infected cells and, therefore, not detectable by virus-specific CTLs. With the use of a new flow cytometric procedure, we show here that a high proportion of naturally infected CD4+ peripheral blood mononuclear cells (PBMC) (between 10% and 80%) are capable of expressing Tax, the Immunodominant target antigen recognized by virus-specific CTLs, Furthermore, we provide direct evidence that autologous CD8+ T cells rapidly kill CD4+ cells naturally infected with HTLV-I and expressing Tax in vitro by a perforin-dependent mechanism, Consistent with these observations, we observed a significant negative correlation between the frequency of Tax(11.19)-specific CD8+ T cells and the percentage of CD4+ T cells in peripheral blood of patients infected with HTLV-I, Those results are in accordance with the view that virus-specific CTLs participate in a highly efficient immune surveillance mechanism that persistently destroys Tax-expressing HTLV-I-infected CD4+ T cells in vivo. (C) 2000 by The American Society of Hematology.
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页码:1386 / 1392
页数:7
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