Effects of cigarette smoking on the human urinary proteome

被引：41

作者：

Airoldi, Luisa ^{[1
]}

Magagnotti, Cinzia ^{[1
]}

Iannuzzi, Angela Rita ^{[1
]}

Marelli, Cristina ^{[1
]}

Bagnati, Renzo ^{[1
]}

Pastorelli, Roberta ^{[1
]}

Colombi, Alessandro ^{[1
]}

Santaguida, Stefano ^{[1
]}

Chiabrando, Chiara ^{[1
]}

Schiarea, Silvia ^{[1
]}

Fanelli, Roberto ^{[1
]}

机构：

[1] Ist Ric Farmacol Mario Negri, Dept Environm Hlth Sci, I-20156 Milan, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 03期

关键词：

2-Dimensional electrophoresis; Cigarette smoking; Mass spectrometry; Pathway analysis; Proteomics; Urine; Western blotting; NF-KAPPA-B; PROTEINS; ACTIVATION; ZINC-ALPHA(2)-GLYCOPROTEIN; INFLAMMATION; SECRETION; PANCREAS; NICOTINE; SERUM;

D O I：

10.1016/j.bbrc.2009.02.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this pilot study we used a proteomic approach to compare the urinary protein patterns of healthy smokers and non-smokers. Proteins were resolved by two-dimensional gel electrophoresis and identified by mass spectrometry. The relative abundance of three inflammatory proteins (S100A8, inter-alpha-trypsin inhibitor heavy chain 4, CD59) and that of two isoforms of pancreatic alpha amylase was significantly higher in smokers. Zinc-alpha-2-glycoprotein was the only protein clown-regulated in smokers. Its abundance was significantly correlated with urinary glucocorticoids. Most of the proteins identified may be non-specific biomarkers of tobacco effects, since they are involved in inflammatory responses associated with several diseases. Of greater interest are the changes in abundance of pancreatic alpha amylase and zinc alpha 2 glycoprotein, which after proper validation, might be candidate biomarkers of diseases resulting from exposure to tobacco smoke. The data also show for the first time that smoking can affect the expression profile Of urinary proteins. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：397 / 402

页数：6

共 25 条

[1] Transcription factor NF-κB -: A sensor for smoke and stress signals [J].

Ahn, KS ;

Aggarwal, BB .

NATURAL PRODUCTS AND MOLECULAR THERAPY, 2005, 1056 :218-233

[2] The relationship between smoking status and cortisol secretion [J].

Badrick, Ellena ;

Kirschbaum, Clemens ;

Kumari, Meena .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2007, 92 (03) :819-824

[3] Zinc-α2-glycoprotein, a lipid mobilizing factor, and is expressed and secreted by human (SGBS) adipocytes [J].

Bao, Y ;

Bing, C ;

Hunter, L ;

Jenkins, JR ;

Wabitsch, M ;

Trayhurn, P .

FEBS LETTERS, 2005, 579 (01) :41-47

[4] Renal albumin absorption in physiology and pathology [J].

Birn, H ;

Christensen, E .

KIDNEY INTERNATIONAL, 2006, 69 (03) :440-449

[5] Activation of p-ERK1/2 by nicotine in pancreatic tumor cell line AR42J: effects on proliferation and secretion [J].

Bose, C ;

Zhang, HL ;

Udupa, KB ;

Chowdhury, P .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2005, 289 (05) :G926-G934

[6] Quantitative measurement of the novel human plasma protein, IHRP, by sandwich ELISA [J].

Choi-Miura, NH .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2001, 24 (03) :214-217

[7] A cell-based approach to study changes in the pancreas following nicotine exposure in an animal model of injury [J].

Chowdhury, Parimal ;

Walker, Azida .

LANGENBECKS ARCHIVES OF SURGERY, 2008, 393 (04) :547-555

[8] Intracellular and extracellular roles of s100 proteins [J].

Donato, R .

MICROSCOPY RESEARCH AND TECHNIQUE, 2003, 60 (06) :540-551

[9] RAISED SERUM CONCENTRATIONS OF PANCREATIC-ENZYMES IN CIGARETTE SMOKERS [J].

DUBICK, MA ;

CONTEAS, CN ;

BILLY, HT ;

MAJUMDAR, APN ;

GEOKAS, MC .

GUT, 1987, 28 (03) :330-335

[10] Zinc α2-glycoprotein:: A multidisciplinary protein [J].

Hassan, Md Imtaiyaz ;

Waheed, Abdul ;

Yadav, Savita ;

Singh, Tej P. ;

Ahmad, Faizan .

MOLECULAR CANCER RESEARCH, 2008, 6 (06) :892-906

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