The H+/ATP coupling ratio of the ATP synthase from thiol-modulated chloroplasts and two cyanobacterial strains is four

被引:96
作者
VanWalraven, HS
Strotmann, H
Schwarz, O
Rumberg, B
机构
[1] UNIV DUSSELDORF,INST BIOCHEM PFLANZEN,D-40225 DUSSELDORF,GERMANY
[2] TECH UNIV BERLIN,MAX VOLMER INST BIOPHYS & PHYS CHEM,D-10623 BERLIN,GERMANY
关键词
ATP synthase; H+-; energy transduction; proton translocation; proton gradient; H+/ATP ratio; enzyme regulation; chloroplasts; cyanobacteria;
D O I
10.1016/0014-5793(95)01536-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this paper the authors emphasise that the proton translocating ATP synthase from thiol-modulated chloroplasts and two cyanobacterial strains has a coupling ratio of 4 protons per ATP synthesised or hydrolysed, This ratio is determined by several thermodynamic studies at equilibrium between phosphate potential (Delta G(p)) and proton gradient (<Delta(mu)over bar> (H+)>), and is confirmed by measurement of proton flux during ATP hydrolysis, Ratios lower than 4 H+/ATP that have been published in the past have predominantly been determined with the oxidised chloroplast enzyme. Errors in these measurements will be discussed.
引用
收藏
页码:309 / 313
页数:5
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