Channelrhodopsin-1: A light-gated proton channel in green algae

被引：764

作者：

Nagel, G

Ollig, D

Fuhrmann, M

Kateriya, S

Mustl, AM

Bamberg, E

Hegemann, P

机构：

[1] Max Planck Inst Biophys, D-60596 Frankfurt, Germany

[2] Univ Regensburg, Inst Biochem, D-93040 Regensburg, Germany

[3] Univ Calabria, Dipartimento Farmacobiol, I-87036 Arcavacata Di Rende, Italy

来源：

SCIENCE | 2002年 / 296卷 / 5577期

关键词：

D O I：

10.1126/science.1072068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.

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页码：2395 / 2398

页数：4

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