Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology

被引：178

作者：

Gao, Hong ^{[1
]}

Liu, Mei ^{[1
]}

Liu, Jintao ^{[1
]}

Dai, Huanqin ^{[1
]}

Zhou, Xianlong ^{[1
]}

Liu, Xiangyang ^{[1
]}

Zhuo, Ying ^{[1
]}

Zhang, Wenquan ^{[1
]}

Zhang, Lixin ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China

[2] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 17期

基金：

中国国家自然科学基金;

关键词：

Streptomyces avermitilis; Avermectin B1a; Medium optimization; Response surface; PHENAZINE-1-CARBOXYLIC ACID PRODUCTION; STATISTICAL-METHODS; HYDROGEN-PRODUCTION; CULTURE-CONDITIONS; BIOSYNTHESIS; COMPONENTS; FERMENTATION; PARAMETERS; STRAIN;

D O I：

10.1016/j.biortech.2009.03.013

中图分类号：

S2 [农业工程];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Response surface methodology was employed to optimize the composition of medium for the production of avermectin B1a by Streptomyces avermitilis 14-12A in shaker flask cultivation. Corn starch and yeast extract were found to have significant effects on avermectin B1a production by the Plackett-Burman design. The steepest ascent method was used to access the optimal region of the medium composition, followed by an application of response surface. The analysis revealed that the optimum values of the tested variables were 149.57 g/l corn starch and 8.92 g/l yeast extract. A production of 5128 mg/l, which was in agreement with the prediction, was observed in verification experiment. In comparison to the production of original level (3528 mg/l), 1.45-fold increase had been obtained. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4012 / 4016

页数：5

共 33 条

[1]

AVERMECTINS, NEW FAMILY OF POTENT ANTHELMINTIC AGENTS - PRODUCING ORGANISM AND FERMENTATION [J].