Blockade of α6-Integrin Reveals Diversity in Homing Patterns Among Human, Baboon, and Murine Cells

被引:8
作者
Bonig, Halvard [1 ,2 ,4 ]
Priestley, Gregory V. [4 ]
Wohlfahrt, Martin [3 ]
Kiem, Hans-Peter [3 ]
Papayannopoulou, Thalia [4 ]
机构
[1] Univ Frankfurt, German Red Cross Blood Ctr, D-60528 Frankfurt, Germany
[2] Univ Frankfurt, Inst Transfus Med & Immunohematol, D-60528 Frankfurt, Germany
[3] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA
[4] Univ Washington, Dept Med, Div Hematol, Seattle, WA 98195 USA
基金
美国国家卫生研究院;
关键词
HEMATOPOIETIC PROGENITOR CELLS; COLONY-STIMULATING FACTOR; BONE-MARROW; NOD/SCID MICE; ADHESION MOLECULES; CD34(+) CELLS; CORD BLOOD; STEM; ENGRAFTMENT; INTEGRINS;
D O I
10.1089/scd.2008.0269
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Our understanding of the mechanisms by which intravenously transplanted hematopoietic stem/progenitor cells (HSPCs) home to and engraft the bone marrow (BM) remains incomplete, but participation of adhesion molecules has been documented. We here demonstrate that blockade of the alpha 6-integrin enhanced BM homing of human and nonhuman primate BM-derived HSPCs by >60% in the xenogeneic transplant model and led to significantly improved engraftment. The effect was limited to BM-derived HSPCs, as granulocyte-colony-stimulating factor mobilized peripheral blood or cord blood HSPCs express little or no alpha 6 integrin. By contrast, despite high alpha 6 integrin expression, no effect of alpha 6 blockade on murine BM-HSPCs homing/engraftment was observed.
引用
收藏
页码:839 / 844
页数:6
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