Genomic convergence toward diploidy in Saccharomyces cerevisiae

被引:172
作者
Gerstein, Aleeza C. [1 ]
Chun, Hye-Jung E. [1 ]
Grant, Alex [1 ]
Otto, Sarah P. [1 ]
机构
[1] Univ British Columbia, Dept Zool, Vancouver, BC V5Z 1M9, Canada
来源
PLOS GENETICS | 2006年 / 2卷 / 09期
关键词
D O I
10.1371/journal.pgen.0020145
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Genome size, a fundamental aspect of any organism, is subject to a variety of mutational and selection pressures. We investigated genome size evolution in haploid, diploid, and tetraploid initially isogenic lines of the yeast Saccharomyces cerevisiae. Over the course of similar to 1,800 generations of mitotic division, we observed convergence toward diploid DNA content in all replicate lines. This convergence was observed in both unstressful and stressful environments, although the rate of convergence was dependent on initial ploidy and evolutionary environment. Comparative genomic hybridization with microarrays revealed nearly euploid DNA content by the end of the experiment. As the vegetative life cycle of S. cerevisiae is predominantly diploid, this experiment provides evidence that genome size evolution is constrained, with selection favouring the genomic content typical of the yeast's evolutionary past.
引用
收藏
页码:1396 / 1401
页数:6
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