Chinese medicine induces neurite outgrowth in PC12 mutant cells incapable of differentiation

被引：6

作者：

Kano, Y ^{[1
]}

Takaguchi, S ^{[1
]}

Nohno, T ^{[1
]}

Hiragami, F ^{[1
]}

Kawamura, K ^{[1
]}

Iwama, MK ^{[1
]}

Miyamoto, K ^{[1
]}

Takehara, M ^{[1
]}

机构：

[1] Kibi Int Univ, Dept Hlth Sci, Okayama 7168508, Japan

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2002年 / 30卷 / 2-3期

关键词：

Chinese medicine; PC12 mutant cells; neurite outgrowth;

D O I：

10.1142/S0192415X02000260

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

During continuous culture of neural PC12 cells, we obtained a drug-hypersensitive PC12 mutant cell that showed high stimulation of neurite outgrowth by various drugs. When several Chinese medicines such as Shu-Jing-Huo-Xie-Tang and Wu-Ling-San were provided to these PC12 mutant cells, the frequency of nerve growth factor (NGF)-induced neurite outgrowth increased approximately 30-fold compared to NGF alone. Neurite outgrowth induced by NGF in PC 12 cells is accompanied by sustained activation of mitogen-activated protein kinase (MAPK); however, these Chinese medicines did not induce MAPK activity. The findings thus indicate that certain Chinese medicines may induce neurite outgrowth by a novel mechanism which is distinct from the NGF-activated pathway in PC 12 mutant cells.

引用

页码：287 / 295

页数：9

共 22 条

[1] Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons [J].