Experimental design considerations in quantification experiments by using the internal standard technique: Cholesterol determination by gas chromatography as a case study

被引：30

作者：

Araujo, P ^{[1
]}

Couillard, F ^{[1
]}

Leirnes, E ^{[1
]}

Ask, K ^{[1
]}

Bokevoll, A ^{[1
]}

Froyland, L ^{[1
]}

机构：

[1] NIFES, N-5817 Bergen, Norway

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1121卷 / 01期

关键词：

internal standard; modelling; experimental design; Doehlert matrix; quantification; cholesterol;

D O I：

10.1016/j.chroma.2006.03.119

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An overview on experimental design in quantification experiments using the internal standard technique is described. Particular emphasis is given to the modelling of the relationship analyte/internal standard and the estimation of the optimal amount of internal standard to be used throughout the analytical concentration range by using a uniform shell design with a minimum number of seven experiments. The described approach is demonstrated in the analysis of cholesterol in various reference materials by gas chromatography (GC), using alpha-5 cholestan as internal standard. The outlined approaches can be applied in the determination of any kind of analyte. (c) 2006 Elsevier B.V All rights reserved.

引用

页码：99 / 105

页数：7

共 10 条

[1]

Visualisation of confidence in two-factor designs where model, replication and star points are varied [J].