Identification and cross-species amplification of EST derived SSR markers in different bamboo species

被引：43

作者：

Sharma, Vikas ^{[1
]}

Bhardwaj, Pankaj ^{[1
]}

Kumar, Rahul ^{[1
]}

Sharma, Ram Kumar ^{[1
]}

Sood, Anil ^{[1
]}

Ahuja, Paramvir Singh ^{[1
]}

机构：

[1] CSIR, IHBT, Div Biotechnol, Inst Himalayan Bioresource Technol, Palampur 176061, Himachal Prades, India

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 03期

关键词：

Bambusa oldhamii; Bamboo; Cross-amplification; EST-SSR; MICROSATELLITES;

D O I：

10.1007/s10592-008-9630-1

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

The availability of expressed sequence data derived from gene discovery programs became an alternative source of mining simple sequence repeat (SSR) and developing inexpensive genetic markers for the crop improvements. In present study, 10 express sequence tags (EST)-SSR markers were identified from Bambusa oldhamii public sequence data base. Transferability to 25 species of Bambusoideae ranged from 30% to 100%. The number of alleles detected per locus ranged from 2 to 10. All the newly identified SSR markers were found to be moderately to highly polymorphic with an average Polymorphic Information Content (PIC) value of 0.54. As these loci represents transcribed region and recorded high level of cross transferability and reliable amplification across the species, demonstrating the utility of these markers for functional and genetic analyses of bamboo species.

引用

页码：721 / 724

页数：4

共 13 条

[1] OPTIMIZING PARENTAL SELECTION FOR GENETIC-LINKAGE MAPS [J].