Dicer independent small RNAs associate with telomeric heterochromatin

被引：51

作者：

Cao, Fang ^{[1
]}

Li, Xiangzhi ^{[1
]}

Hiew, Samantha ^{[3
]}

Brady, Hugh ^{[4
]}

Liu, Yifan ^{[1
]}

Dou, Yali ^{[1
,2
]}

机构：

[1] Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Biol Chem, Ann Arbor, MI 48109 USA

[3] UCL, Inst Child Hlth, Mol Haematol & Canc Biol Unit, London WC1N 1EH, England

[4] Univ London Imperial Coll Sci Technol & Med, Immunol & Infect Sect, Div Cell & Mol Biol, London SW7 2AZ, England

来源：

RNA | 2009年 / 15卷 / 07期

关键词：

heterochromatin assembly; histone methylation; piRNA; HISTONE-H3; LYSINE-9; METHYLATION; REPEAT-CONTAINING RNA; GENOME REARRANGEMENT; STEM-CELLS; EPIGENETIC REGULATION; MAMMALIAN TELOMERES; DNA ELIMINATION; PROTEIN; COMPLEX; LENGTH;

D O I：

10.1261/rna.1423309

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small RNAs play important roles in the establishment and maintenance of heterochromatin structures. We show the presence of telomere specific small RNAs (tel-sRNAs) in mouse embryonic stem cells that are similar to 24 nucleotides in length, Dicer-independent, and 2'-O-methylated at the 3' terminus. The tel-sRNAs are asymmetric with specificity toward telomere G-rich strand, and evolutionarily conserved from protozoan to mammalian cells. Furthermore, tel-sRNAs are up-regulated in cells that carry null mutation of H3K4 methyltransferase MLL (Mll((-/-))) and down-regulated in cells that carry null mutations of histone H3K9 methyltransferase SUV39H (Suv39h1/h2((-/-))), suggesting that they are subject to epigenetic regulation. These results support that tel-sRNAs are heterochromatin associated pi-like small RNAs.

引用

页码：1274 / 1281

页数：8

共 45 条

[1] A novel class of small RNAs bind to MILI protein in mouse testes [J].