Identification of the hepatitis C virus E2 glycoprotein binding site on the large extracellular loop of CD81

被引：104

作者：

Drummer, HE ^{[1
]}

Wilson, KA ^{[1
]}

Poumbourios, P ^{[1
]}

机构：

[1] St Vincents Inst Med Res, Fitzroy, Vic 3065, Australia

来源：

JOURNAL OF VIROLOGY | 2002年 / 76卷 / 21期

关键词：

D O I：

10.1128/JVI.76.21.11143-11147.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The binding of hepatitis C virus glycoprotein E2 to the large extracellular loop (LEL) of CD81 has been shown to modulate human T-cell and NK cell activity in vitro. Using random mutagenesis of a chimera of maltose-binding protein and LEL residues 113 to 201, we have determined that the E2-binding site on CD81 comprises residues Ile(182,) Phe(186), Asn(184), and Len(162). These findings reveal an E2-binding surface of approximately 806 Angstrom(2) and potential target sites for the development of small-molecule inhibitors of E2 binding.

引用

页码：11143 / 11147

页数：5

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