Matrix metalloproteinase-8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients

被引:98
作者
Chen, HY
Cox, SW
Eley, BM
Mäntylä, P
Rönkä, H
Sorsa, T
机构
[1] Guys Kings & St Thomas Dent Inst, Dept Periodontol, London SE5 9RW, England
[2] Beijing Med Univ, Clin Sch 3, Dept Stomatol, Beijing 100083, Peoples R China
[3] Univ Helsinki, Inst Dent, Dept Periodontol, Helsinki, Finland
[4] Oy Ale, Medix Biochem, Kauniainen, Finland
关键词
gingival crevicular fluid; periodontitis; matrix metalloproteinase-8; collagenase; elastase;
D O I
10.1034/j.1600-051x.2000.027005366.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Aim: To make an initial assessment of the periodontal diagnostic potential of immunoreactive matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF) by comparison with elastase activity which has previously been associated with disease severity and progression. Methods: GCF was collected from molar and premolar sites of 16 chronic adult periodontitis patients before treatment and 13 of this group 2 weeks after scaling and root planing. Samples were analysed for MMP-8 by immunofluorometric assay and for elastase activity with a fluorogenic substrate. Results: Mean patient clinical parameters and GCF enzyme totals both decreased significantly after treatment. Total MMP-8 levels and elastase activities generally correlated significantly with gingival and bleeding indices. For GCF concentrations, only MMP-8 showed a significant fall after treatment, and some significant correlations with clinical parameters. Amounts of the 2 enzymes correlated significantly with each other. Conclusions: Similarities between MMP-8 and elastase probably reflect the fact that both enzymes are associated mainly with neutrophils: MMP-8 levels may have fallen more after treatment because the assay, unlike that for elastase, would most likely not have detected much enzyme bound to alpha(2)-macroglobulin. The immunoassay for MMP-8 is more specific and convenient than functional collagenase assays, and might be suitable for monitoring the periodontal condition.
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页码:366 / 369
页数:4
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