Determination of protein-ligand association thermochemistry using variable-temperature nanoelectrospray mass spectrometry

被引：62

作者：

Daneshfar, R ^{[1
]}

Kitova, EN ^{[1
]}

Klassen, JS ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 15期

关键词：

D O I：

10.1021/ja0316972

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel temperature-controlled nanoelectrospray (nanoES) device, interfaced with a Fourier transform ion cyclotron resonance mass spectrometer, is used to measure the association constants, Kassoc, for a series of protein-carbohydrate complexes at solution temperatures ranging from 5 to 40 °C. From a van't Hoff analysis of the Kassoc values, the enthalpies and entropies of association (ΔHassoc, ΔSassoc) at 25 °C are determined. The nanoES/mass spectrometry-derived thermodynamic parameters are in agreement with values previously determined by isothermal titration calorimetry. Copyright © 2004 American Chemical Society.

引用

页码：4786 / 4787

页数：2

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