Immobilisation studies and catalytic properties of microbial lipase onto styrene-divinylbenzene copolymer

被引：92

作者：

de Oliveira, PC ^{[1
]}

Alves, GM ^{[1
]}

de Castro, HF ^{[1
]}

机构：

[1] Fac Chem Engn Lorena, Dept Chem Engn, BR-12600000 Lorena, SP, Brazil

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2000年 / 5卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

lipase; styrene-divinylbenzene copolymer; aqueous and non-aqueous environment; hydrolytic and esterification activities;

D O I：

10.1016/S1369-703X(99)00061-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial lipase from Candida rugosa was immobilised by physical adsorption onto styrene-divinylbenzene (STYDVB) copolymer. Two different coupling media were evaluated: aqueous solution (sodium phosphate buffer) and organic solvent (heptane). The choice of the immobilisation medium had a large effect on the hydrolytic and synthetic lipase activities. Better results were found when the coupling procedure was performed in the presence of heptane. The effects of enzyme loading and support capacity regeneration were also studied. Both, free and immobilised lipases were characterised by determining the activity profile as a function of pH, temperature and thermal stability. For the immobilised lipase, operational and storage stabilities were also determined. Operational stability tests indicated that a small enzyme deactivation occurs after 12 consecutive batches of 24 h each. Such results revealed good potential for recycling under nonaqueous system. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：63 / 71

页数：9

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