Toll-like receptor 2 is important for the TH1 response to cutaneous sensitization

被引:64
作者
Jin, Haoli [1 ,2 ]
Kumar, Lalit [1 ,2 ]
Mathias, Clinton [1 ,2 ]
Zurakowski, David [3 ]
Oettgen, Hans [1 ,2 ]
Gorelik, Leonid [4 ]
Geha, Raif [1 ,2 ]
机构
[1] Childrens Hosp, Div Immunol, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA
[3] Childrens Hosp, Dept Anesthesia & Surg, Boston, MA 02115 USA
[4] Biogen Idec Inc, Cambridge, MA USA
基金
美国国家卫生研究院;
关键词
Toll-like receptor 2; atopic dermatitis; contact hypersensitivity; oxazolone; EOSINOPHIL-DERIVED NEUROTOXIN; ATOPIC-DERMATITIS; DENDRITIC CELLS; CYTOKINE/CHEMOKINE PRODUCTION; INTERFERON-GAMMA; CUTTING EDGE; MOUSE MODEL; IFN-GAMMA; KAPPA-B; TLR2;
D O I
10.1016/j.jaci.2009.02.007
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Atopic dermatitis and allergic contact dermatitis are skin disorders triggered by epicutaneous sensitization with protein antigens and contact sensitization with haptens, respectively. Skin is colonized with bacteria, which are a source of Toll-like receptor (TLR) 2 ligands. Objective: We sought to examine the role of TLR2 in marine models of atopic dermatitis and allergic contact dermatitis. Methods: TLR2(-/-) mice and wild-type littermates were epicutaneously sensitized with ovalbumin (OVA) or contact sensitized with oxazolone (OX). Skin histology was assessed by means of hematoxylin and eosin staining and immunohistochemistry. Ear swelling was measured with a micrometer. Cytokine mRNA expression was examined by means of quantitative RT-PCR. Antibody levels and splenocyte secretion of cytokines in response to OVA stimulation were measured by means of ELISA. Dendritic cells were examined for their ability to polarize T-cell receptor/OVA transgenic naive T cells to T(H)1 and T(H)2. Results: In response to OVA sensitization, TLR2(-/-) mice experienced skin infiltration with eosinophils and CD4(+) cells, as well as upregulation of T(H)2 cytokine mRNAs that was comparable with that seen in wild-type littermates. In contrast, epidermal thickening, IFN-gamma expression in the skin, IFN-gamma production by splenocytes, and IgG2a anti-OVA antibody levels were impaired in TLR2(-/-) mice. After OX ear challenge, contact sensitized TLR2(-/-) mice exhibited defective ear swelling with impaired cellular infiltration, decreased epidermal thickening and local IFN-gamma expression, and impaired OX-specific IgG2a responses. Dendritic cells from TLR2(-/-) mice induced significantly lower production of IFN-gamma but normal IL-4 and IL-13 production in naive T cells. Conclusions: These results indicate that TLR2 promotes the IFN-gamma response to cutaneously introduced antigens. (J Allergy Clin Immunol 2009;123:875-82.)
引用
收藏
页码:875 / 882
页数:8
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