Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect

被引：27

作者：

Lukyanov, K

Diatchenko, L

Chenchik, A

Nanisetti, A

Siebert, P

Usman, N

Matz, M

Lukyanov, S

机构：

[1] RUSSIAN ACAD SCI, SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM, MOSCOW 117871, RUSSIA

[2] CLONTECH LABS INC, PALO ALTO, CA 94303 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 230卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.5948

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a method for preparing high-quality cDNA libraries from total RNA. By this method, double-stranded (ds) cDNA ligated with a specially designed ds adaptor is amplified by PCR using a modified T-primer and another primer corresponding to the outer part of the adaptor, The suppression PCR effect strongly inhibits the amplification of poly(A)- RNA, thereby reducing background, This method leads to amplification of high-quality cDNA, facilitating the construction of representative cDNA libraries from as little as 10-100 ng of total RNA. (C) 1997 Academic Press

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收藏

页码：285 / 288

页数：4

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