Phytochemical and genetic analysis in selected chemotypes of Withania somnifera

被引：66

作者：

Dhar, Rekha S.

Verma, Vijeshwar

Suri, Krishan A.

Sangwan, Rajinder S.

Satti, Naresh K.

Kumar, Arun

Tuli, Rakesh

Qazi, Gulam N.

机构：

[1] Reg Res Lab, Div Biotechnol, Jammu 180001, Tawi, India

[2] Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India

[3] Natl Bot Res Inst, Lucknow 226001, Uttar Pradesh, India

来源：

PHYTOCHEMISTRY | 2006年 / 67卷 / 20期

关键词：

Withania somnifera; AFLP; TLC; HPLC; IP; NMR; MS;

D O I：

10.1016/j.phytochem.2006.07.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The main active components and genetic profile of 15 selected accessions of Withania somnifera Dunal. were analysed. Ethanolic extract of the dried roots/leaves of the plant was concentrated under pressure at 50 +/- 5 degrees C and was analysed for main compounds (withanolides and withaferin A) by HPLC. All the main components were found to be present in accessions (AGB 002, AGB 009, RSS 009, RSS 033). Correlation of these main components with their genetic factors, was undertaken using AFLP (amplified fragment length polymorphism) markers. Among 64 primers 7 yielded optimum polymorphism. A total of 913 polymorphic peaks were generated using these primers. Jaccard's similarity coefficient indicated that accessions having almost the same active compounds clustered together. The present study demonstrates that AFLP can be successfully used to resolve the correlation of AFLP data with the presence of secondary metabolites. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2269 / 2276

页数：8

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