Directed evolution to investigate steric control of enzymatic oxidosqualene cyclization. An isoleucine-to-valine mutation in cycloartenol synthase allows lanosterol and parkeol biosynthesis

被引：84

作者：

Hart, EA

Hua, L

Darr, LB

Wilson, WK

Pang, JH

Matsuda, SPT ^{[1
]}

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77005 USA

[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1999年 / 121卷 / 42期

关键词：

D O I：

10.1021/ja992589b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

引用

页码：9887 / 9888

页数：2

共 26 条

[1] ENZYMATIC CYCLIZATION OF SQUALENE AND OXIDOSQUALENE TO STEROLS AND TRITERPENES [J].

ABE, I ;

ROHMER, M ;

PRESTWICH, GD .

CHEMICAL REVIEWS, 1993, 93 (06) :2189-2206

[2] A METHOD FOR GENE DISRUPTION THAT ALLOWS REPEATED USE OF URA3 SELECTION IN THE CONSTRUCTION OF MULTIPLY DISRUPTED YEAST STRAINS [J].

ALANI, E ;

CAO, L ;

KLECKNER, N .

GENETICS, 1987, 116 (04) :541-545

[3]

BOEKE JD, 1987, METHOD ENZYMOL, V154, P164

[4] MULTIFUNCTIONAL YEAST HIGH-COPY-NUMBER SHUTTLE VECTORS [J].

CHRISTIANSON, TW ;

SIKORSKI, RS ;

DANTE, M ;

SHERO, JH ;

HIETER, P .

GENE, 1992, 110 (01) :119-122

[5] ISOLATION OF AN ARABIDOPSIS-THALIANA GENE ENCODING CYCLOARTENOL SYNTHASE BY FUNCTIONAL EXPRESSION IN A YEAST MUTANT LACKING LANOSTEROL SYNTHASE BY THE USE OF A CHROMATOGRAPHIC SCREEN [J].

COREY, EJ ;

MATSUDA, SPT ;

BARTEL, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :11628-11632

[6] Molecular cloning of a Schizosaccharomyces pombe cDNA encoding lanosterol synthase and investigation of conserved tryptophan residues [J].

Corey, EJ ;

Matsuda, SPT ;

Baker, CH ;

Ting, AY ;

Cheng, HM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 219 (02) :327-331

[7] MOLECULAR-CLONING, CHARACTERIZATION, AND OVEREXPRESSION OF ERG7 THE SACCHAROMYCES-CEREVISIAE GENE ENCODING LANOSTEROL SYNTHASE [J].

COREY, EJ ;

MATSUDA, SPT ;

BARTEL, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2211-2215

[8] Studies on the substrate binding segments and catalytic action of lanosterol synthase. Affinity labeling with carbocations derived from mechanism-based analogs of 2,3-oxidosqualene and site-directed mutagenesis probes [J].

Corey, EJ ;

Cheng, HM ;

Baker, CH ;

Matsuda, SPT ;

Li, D ;

Song, XL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (06) :1289-1296

[9] H-1 AND C-13 NMR ASSIGNMENTS FOR LANOSTAN-3 BETA-OL DERIVATIVES - REVISED ASSIGNMENTS FOR LANOSTEROL [J].

EMMONS, GT ;

WILSON, WK ;

SCHROEPFER, GJ .

MAGNETIC RESONANCE IN CHEMISTRY, 1989, 27 (11) :1012-1024

[10]

HEINTZ R, 1974, J BIOL CHEM, V249, P4267

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