Serological evidence for recent exposure to Taenia solium in Venezuelan Amerindians

被引:18
作者
Ferrer, E
Cortez, MM
Perez, H
De la Rosa, M
De Noya, BA
Dávila, I
Harrison, LJS [1 ]
Foster-Cuevas, M
Parkhouse, RME
Cabrera, A
机构
[1] Univ Edinburgh, Sir Alexander Robertston Ctr Trop Vet Med, Dept Trop Anim Hlth, Roslin EH25 9RG, Midlothian, Scotland
[2] UCV, Inst Trop Med, Caracas, Venezuela
[3] Univ Carabobo, Ctr Invest Biomed, Maracay, Venezuela
[4] Univ Carabobo, Dept Parasitol, Maracay, Venezuela
[5] IVIC, Ctr Microbiol & Biol Celular, Caracas, Venezuela
[6] Inst Anim Hlth, Pirbright Lab, Surrey GU24 0NF, England
[7] Gulbenkian Inst Sci, P-2780155 Oeiras, Portugal
关键词
D O I
10.4269/ajtmh.2002.66.170
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
This study examined the seroprevalence and serum antibody isotype profile for Toenia solium cysticercosis in an Amerindian community in the Amazonas state of Venezuela. An antigen-trapping enzyme-linked immunosorbent assay (Ag-ELISA) was used to detect viable cysticercosis, Indirect ELISA (Ab-ELISA) and enzyme-linked immunoelectrotransfer blot (EITB) was performed by using antigens prepared from T. solium metacestodes to detect anti-parasite antibodies. The Ag-ELISA and Ab-ELISAs revealed 64.7% and 79.0% seropositivity, respectively. in the Amerindian population. Immunoglobulin (Ig) M was the predominant antibody class, suggesting recent infection, In comparison sera from, clinically defined, hospital neurocysticercosis cases revealed only 27% seropositivity by Ag-ELISA, compared with 86-92% seropositivity by Ab-ELISA, and IgG(4) was the predominant antibody subclass detected. The EITB antigen recognition patterns of the hospitalized patients were very similar to that of the Amer-indians, confirming exposure to the parasite. These results. combined with the predominance of IgM antibody responses and the marked detection of secreted products of viable parasites. strongly suggest that recent exposure to T. solium had occurred in the Amerindian population.
引用
收藏
页码:170 / 174
页数:5
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