Solid Lipid Nanoparticles for Potential Doxorubicin Delivery in Glioblastoma Treatment: Preliminary In Vitro Studies

被引：86

作者：

Battaglia, Luigi ^{[1
]}

Gallarate, Marina ^{[1
]}

Peira, Elena ^{[1
]}

Chirio, Daniela ^{[1
]}

Muntoni, Elisabetta ^{[1
]}

Biasibetti, Elena ^{[2
]}

Capucchio, Maria Teresa ^{[2
]}

Valazza, Alberto ^{[2
]}

Panciani, Pier Paolo ^{[3
]}

Lanotte, Michele ^{[3
]}

Schiffer, Davide ^{[4
]}

Annovazzi, Laura ^{[4
]}

Caldera, Valentina ^{[4
]}

Mellai, Marta ^{[4
]}

Riganti, Chiara ^{[5
]}

机构：

[1] Univ Turin, Dipartimento Sci & Tecnol Farmaco, Turin, Italy

[2] Univ Turin, Dipartimento Sci Vet, Grugliasco, Italy

[3] Univ Turin, Dipartimento Neurosci, Turin, Italy

[4] Policlin Monza, Ctr NeuroBioOncol, Vercelli, Italy

[5] Univ Turin, Dipartimento Oncol, Orbassano, Italy

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 2014年 / 103卷 / 07期

关键词：

SLN; coacervation; nanoparticles; doxorubicin; ion pairing; blood-brain barrier; glioblastoma; CNS; BLOOD-BRAIN-BARRIER; PEGYLATED LIPOSOMAL DOXORUBICIN; REGULATES P-GLYCOPROTEIN; DRUG-DELIVERY; TEMOZOLOMIDE; CHEMOTHERAPY; EXPRESSION; TRANSPORT; CELLS; SLN;

D O I：

10.1002/jps.24002

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

The major obstacle to glioblastoma pharmacological therapy is the overcoming of the blood-brain barrier (BBB). In literature, several strategies have been proposed to overcome the BBB: in this experimental work, solid lipid nanoparticles (SLN), prepared according to fatty acid coacervation technique, are proposed as the vehicle for doxorubicin (Dox), to enhance its permeation through an artificial model of BBB. The in vitro cytotoxicity of Dox-loaded SLN has been measured on three different commercial and patient-derived glioma cell lines. Dox was entrapped within SLN thanks to hydrophobic ion pairing with negatively charged surfactants, used as counterions. Results indicate that Dox entrapped in SLN maintains its cytotoxic activity toward glioma cell lines; moreover, its permeation through hCMEC/D3 cell monolayer, assumed as a model of the BBB, was increased when the drug was entrapped in SLN. In conclusion, SLN proved to be a promising vehicle for the delivery of Dox to the brain in glioblastoma treatment. (c) 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:2157-2165, 2014

引用

页码：2157 / 2165

页数：9

共 47 条

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