Langmuir aggregation of chromophore in biomacromolecule and its application: Interaction of picramine CA (PCA) with proteins

被引：14

作者：

Gao, HW ^{[1
]}

Yang, JX ^{[1
]}

Jiang, J ^{[1
]}

Yu, LQ ^{[1
]}

机构：

[1] Anhui Univ, Sch Chem & Chem Engn, Hefei 230039, Peoples R China

来源：

SUPRAMOLECULAR CHEMISTRY | 2002年 / 14卷 / 04期

关键词：

MPASC technique; protein; picramine CA (PCA); microelectrostatic field; Langmuir aggregation;

D O I：

10.1080/10610270290029380

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The microphase adsorption-spectral correction (MPASC) technique has been described. The formation of microelectrostatic fields in macromolecule is proposed and it causes the aggregation of a chromophore in protein. We have studied the interaction of picramine CA (PCA) with five proteins: bovine serum albumin (BSA), human gamma-globulin (gamma-G), bovine hemoglobin (Hb), horse myoglobin (Mb) and ovalbumin (OVA). Results show that at pH 2.21, the adsorption ratios of PCA to BSA, gamma-G, Hb, Mb and OVA are 46, 54, 35, 15 and 21, respectively, their adsorption constants 3.17 x 10(5), 6.58 x 10(4), 1.40 x 10(5), 8.99 x 10(4) and 1.68 x 10(5) and their absorptivities 3.56 x 10(5), 3.06 x 10(5), 2.91 x 10(5), 1.10 x 10(5) and 1.97 x 10(5) 1 mol(-1) cm(-1) at 630 nm. The analysis of samples has given the satisfactory result.

引用

页码：315 / 321

页数：7

共 13 条

[1] Analysis of biosensor chips for identification of nucleic acids [J].

Arlinghaus, HF ;

Kwoka, MN ;

Jacobson, KB .

ANALYTICAL CHEMISTRY, 1997, 69 (18) :3747-3753

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

Gao HW, 2000, J AOAC INT, V83, P231

[4] Study of the spectrophotometric analysis of protein solution with p-iodochlorophosphonazo as adsorbate by the microphase adsorption-spectral correction technique [J].

Gao, HW ;

Jiang, J ;

Yu, LQ .

ANALYST, 2001, 126 (04) :528-533

[5]

GAO HW, 1995, RECL TRAV CHIM PAY B, V114, P61

[6]

Huang CZ, 1998, ANALYST, V123, P1401

[7]

LAEAREV AI, 1975, ZAVODSK LAB, V41, P534

[8] THE ADSORPTION OF GASES ON PLANE SURFACES OF GLASS, MICA AND PLATINUM. [J].

Langmuir, Irving .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1918, 40 :1361-1403

[9] COMPUTER APPROACH TO CONTINUOUS VARIATIONS METHOD FOR SPECTROPHOTOMETRIC DETERMINATION OF EXTRACTION AND FORMATION CONSTANTS [J].

LIKUSSAR, W .

ANALYTICAL CHEMISTRY, 1973, 45 (11) :1926-1931

[10] PORPHYRIN ASSEMBLIES ON DNA AS STUDIED BY A RESONANCE LIGHT-SCATTERING TECHNIQUE [J].

PASTERNACK, RF ;

BUSTAMANTE, C ;

COLLINGS, PJ ;

GIANNETTO, A ;

GIBBS, EJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (13) :5393-5399

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