Electrospray-mass spectrometry characterization and measurement of far-UV-induced thymine photoproducts

被引：59

作者：

Douki, T ^{[1
]}

Court, M ^{[1
]}

Cadet, J ^{[1
]}

机构：

[1] CEA, Dept Rech Fondamentale Mat Condensee, Serv Chim Inorgan & Biol, Lab Les Acides Nucl, F-38054 Grenoble 9, France

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2000年 / 54卷 / 2-3期

关键词：

DNA damage; cyclobutane dimers; (6-4) photoproducts; spore photoproducts;

D O I：

10.1016/S1011-1344(00)00009-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Far-UV-induced formation of dimeric pyrimidine photoproducts within DNA is a major cause of the carcinogenic effects of solar light. The chemical structure of this class of lesion has been mostly determined by studies on model compounds. The present work is aimed at providing mass spectrometry data on the thymine-thymine photoproducts, including the diastereoisomers of the cyclobutane dimer, the (6-4) adduct, the related Dewar valence isomer and the spore photoproduct. Fragmentation mass spectra of the modified bases, nucleosides, dinucleoside monophosphates and dinucleotides were recorded following electrospray ionization with either triple-quadrupolar or ion-trap detection. The results showed differences in fragmentation pattern between the different types of photoproducts. In addition, a drastic effect of the diastereoisometry was observed for the cyclobutane dimers. A sensitive detection technique has been developed for the analysis of dinucleoside monophosphate photoproducts by high-performance liquid chromatography associated with mass spectrometry in the negative mode with multiple reaction-monitoring detection. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：145 / 154

页数：10

共 39 条

[1] THERMOSPRAY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MASS-SPECTRAL ANALYSES OF THE PHOTOPRODUCTS OF DTPDT AND DTPDU [J].