Response of dairy cows fed grass silage diets to abomasal infusions of histidine alone or in combinations with methionine and lysine

The response of dairy cows fed grass silage-based diets to the abomasal infusion of water (control) or 6.5 g of His alone or in combination with either 6.0 g of Met or 19.0 g of Lys or both was studied in an incomplete 4 x 5 Latin square experiment with 14-d periods. Each cow received a basal diet of 8 kg/d of cereal concentrate [12.1% crude protein (CP)] and free access to grass silage (14.1% CP) ensiled with an acid-based additive. Postruminal infusions increased arterial plasma concentrations of the amino acids CAA) infused, but-compared with control, only the infusion of His (18 vs. 57 mu mol/L) was associated with significant increases in milk and milk protein yields. Infusions of His did not affect dry matter intake of grass silage, rumen fermentation, or diet digestibility. Milk protein content was unchanged by treatments, but His infusions decreased lactose and fat contents. The combinations of AA did not produce any further responses compared with His alone. However, milk protein percentage was slightly higher, and milk fat percentage tended to-be higher when Met rather than Lys was infused with His. We concluded that His is the first-limiting AA when grass silage-based diets are supplemented with cereal concentrates, while neither Met nor Lys are the second-limiting AA with grass silage feeding.