Critical evaluation of the applicability of capillary zone electrophoresis for the study of hapten-antibody complex formation

被引：32

作者：

Busch, MHA ^{[1
]}

Boelens, HFM ^{[1
]}

Kraak, JC ^{[1
]}

Poppe, H ^{[1
]}

Meekel, AAP ^{[1
]}

Resmini, M ^{[1
]}

机构：

[1] UNIV AMSTERDAM,ORGAN CHEM LAB,AMSTERDAM INST MOL STUDIES,NL-1018 WS AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 744卷 / 1-2期

关键词：

affinity capillary electrophoresis; association constants; frontal analysis; antibodies; haptens;

D O I：

10.1016/0021-9673(96)00275-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The applicability of capillary zone electrophoresis (CZE) for the determination of association constants of an hapten-antibody complex with values as high as 10(7) (mol/l)(-1) was investigated (dissociation constant in the nmol range). As a reference method the well known, enzyme-linked immunosorbent assay (ELISA) was selected. The study describes the optimisation of the experimental conditions of the CZE technique. The CZE measurements were optimised according to an experimental design. The results of the CZE and ELISA methods are compared giving consideration to the reproducibility (repeatability) of the two methods.

引用

页码：195 / 203

页数：9

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