3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers

被引：130

作者：

Du, Mingchun ^{[1
,2
]}

Chen, Bing ^{[1
]}

Meng, Qingyuan ^{[1
]}

Liu, Sumei ^{[1
]}

Zheng, Xiongfei ^{[3
]}

Zhang, Cheng ^{[3
]}

Wang, Heran ^{[3
]}

Li, Hongyi ^{[3
]}

Wang, Nuo ^{[1
]}

Dai, Jianwu ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100190, Peoples R China

[2] ZonHon Biopharma Inst Inc, Changzhou 213022, Peoples R China

[3] Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China

来源：

BIOFABRICATION | 2015年 / 7卷 / 04期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

3D bioprinting; BMSC; methacrylamide gelatin scaffold; CBD-BMP2-collagen microfiber; osteogenic differentiation; MESENCHYMAL STEM-CELLS; BONE MORPHOGENETIC PROTEIN-2; FIBROBLAST-GROWTH-FACTOR; CROSS-LINKING HEPARIN; COLLAGEN SCAFFOLDS; EXTRACELLULAR-MATRIX; ENGINEERED CARTILAGE; CHONDROITIN SULFATE; TISSUE; REGENERATION;

D O I：

10.1088/1758-5090/7/4/044104

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Three-dimensional (3D) bioprinting combines biomaterials, cells and functional components into complex living tissues. Herein, we assembled function-control modules into cell-laden scaffolds using 3D bioprinting. A customized 3D printer was able to tune the microstructure of printed bone mesenchymal stem cell (BMSC)-laden methacrylamide gelatin scaffolds at the micrometer scale. For example, the pore size was adjusted to 282 +/- 32 mu m and 363 +/- 60 mu m. To match the requirements of the printing nozzle, collagen microfibers with a length of 22 +/- 13 mu m were prepared with a highspeed crusher. Collagen microfibers bound bone morphogenetic protein 2 (BMP2) with a collagen binding domain (CBD) as differentiation-control module, from which BMP2 was able to be controllably released. The differentiation behaviors of BMSCs in the printed scaffolds were compared in three microenvironments: samples without CBD-BMP2-collagen microfibers in the growth medium, samples without microfibers in the osteogenic medium and samples with microfibers in the growth medium. The results indicated that BMSCs showed high cell viability (>90%) during printing; CBD-BMP2-collagen microfibers induced BMSC differentiation into osteocytes within 14 days more efficiently than the osteogenic medium. Our studies suggest that these function-control modules are attractive biomaterials and have potential applications in 3D bioprinting.

引用

页数：10

共 62 条

[1]

Bone regeneration, cell therapy and tissue engineering [J].