Listeria monocytogenes isolated from cold-smoked fish products in Osaka City, Japan

被引:34
作者
Nakamura, H [1 ]
Hatanaka, M
Ochi, K
Nagao, M
Ogasawara, J
Hase, A
Kitase, T
Haruki, K
Nishikawa, Y
机构
[1] Osaka City Univ, Grad Sch Human Life Sci, Dept Food & Human Hlth Sci, Osaka 5588585, Japan
[2] Osaka City Inst Publ Hlth & Environm Sci, Dept Microbiol, Osaka 5430026, Japan
关键词
listeria monocytogenes; seafood; cold-smoked fish; culture media; prevalence; molecular epidemiology;
D O I
10.1016/j.ijfoodmicro.2004.02.010
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Listeria monocytogenes contamination of ready-to-eat seafood products commercially available in Osaka was examined between 1999 and 2000. L. monocytogenes was isolated from 12 (13%) of the 95 products tested. All positive samples were from cold-smoked fish with 9 being obtained during the summer. Thirteen isolates of L. monocytogenes were typed by pulsed-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR)-based typing methods. Isolates of the same serotype originating from the same manufacturer gave similar DNA profiles, irrespective of the type of sample or date of isolation. The finding suggest that persistent strains in each manufacturing facility proliferate during the summer and contaminate products during manufacturing processes. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:323 / 328
页数:6
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