Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin

被引：41

作者：

Malicka, J ^{[1
]}

Gryczynski, I ^{[1
]}

Kusba, J ^{[1
]}

Shen, YB ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] Univ Maryland, Ctr Fluorescence, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 294卷 / 04期

关键词：

D O I：

10.1016/S0006-291X(02)00555-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 Angstrom. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to it bound probe as the acceptor. These preliminary experiments revealed a dramatic increase in the apparent Forster distance increasing from 28.6 to 63 Angstrom. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：886 / 892

页数：7

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