Evidence for cyclooxygenase-1 association with caveolin-1 and-2 in cultured human embryonic kidney (HEK 293) cells

被引：16

作者：

Cha, SH ^{[1
]}

Jung, NH ^{[1
]}

Kim, BR ^{[1
]}

Kim, HW ^{[1
]}

Kwak, JO ^{[1
]}

机构：

[1] Inha Univ, Coll Med, Dept Pharmacol & Toxicol, Inchon 382751, South Korea

来源：

IUBMB LIFE | 2004年 / 56卷 / 04期

关键词：

HEK; 293; cell; cyclooxygenase-1; caveolin;

D O I：

10.1080/15216540410001699312

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this study was to confirm protein-protein interaction between cyclooxygenase-1 (COX-1) and caveolins. The interaction of cyclooxygenase-1 and caveolins in the cultured human embryonic kidney (HEK 293) cells was investigated using immuno-precipitation and Western blot analysis. In HEK 293 cells, high levels of caveolin-2 and low level of caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Caveolae rich membranous fractions from the HEK 293 cells contained both COX-1 and caveolin-1 or caveolin-2 in same fractions. The experiments of immuno-precipitation showed complex formation between the COX-1 and caveolin-1 or caveolin-2 in the HEK 293 cells. Confocal microscopic results also support co-localization of COX-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with cylooxygenase-1 in caveolae suggested that caveolin would play an important role in regulating the function of COX-1.

引用

页码：221 / 227

页数：7

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