Kinesin superfamily protein 3 (KIF3) motor transports fodrin-associating vesicles important for neurite building

被引:160
作者
Takeda, S [1 ]
Yamazaki, H [1 ]
Seog, DH [1 ]
Kanai, Y [1 ]
Terada, S [1 ]
Hirokawa, N [1 ]
机构
[1] Univ Tokyo, Grad Sch Med, Dept Anat & Cell Biol, Bunkyo Ku, Tokyo 1130033, Japan
关键词
kinesin superfamily protein 3; fodrin; microinjection; axonal transport; yeast two-hybrid;
D O I
10.1083/jcb.148.6.1255
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Kinesin superfamily proteins (KIFs) comprise several dozen molecular motor proteins. The KIF3 heterotrimer complex is one of the most abundantly and ubiquitously expressed KIFs in mammalian cells. To unveil the functions of KIF3, microinjection of function-blocking monovalent antibodies against KIF3 into cultured superior cervical ganglion (SCG) neurons was carried out,They significantly blocked fast axonal transport and brought about inhibition of neurite extension. A yeast two-hybrid binding assay revealed the association of fodrin with the KIF3 motor through KAP3. This was further confirmed by using vesicles collected from large bundles of axons (cauda equina), from which membranous vesicles could be prepared in pure preparations. Both immunoprecipitation and immunoelectron microscopy indicated the colocalization of fodrin and KIF3 on the same vesicles, the results reinforcing the evidence that the cargo of the KIF3 motor consists of fodrin-associating vesicles. In addition, pulse-labeling study implied partial comigration of both molecules as fast flow components. Taken together, the KIF3 motor is engaged in fast axonal transport that conveys membranous components important for neurite extension.
引用
收藏
页码:1255 / 1265
页数:11
相关论文
共 56 条
[1]   KINESIN FAMILY IN MURINE CENTRAL-NERVOUS-SYSTEM [J].
AIZAWA, H ;
SEKINE, Y ;
TAKEMURA, R ;
ZHANG, ZZ ;
NANGAKU, M ;
HIROKAWA, N .
JOURNAL OF CELL BIOLOGY, 1992, 119 (05) :1287-1296
[2]  
Ausubel F.A., 1997, CURRENT PROTOCOLS MO, DOI DOI 10.1.4
[3]   ASSEMBLY OF MICROTUBULES AT THE TIP OF GROWING AXONS [J].
BAMBURG, JR ;
BRAY, D ;
CHAPMAN, K .
NATURE, 1986, 321 (6072) :788-790
[4]  
Beech PL, 1996, J CELL SCI, V109, P889
[5]   A MONOCLONAL-ANTIBODY AGAINST KINESIN INHIBITS BOTH ANTEROGRADE AND RETROGRADE FAST AXONAL-TRANSPORT IN SQUID AXOPLASM [J].
BRADY, ST ;
PFISTER, KK ;
BLOOM, GS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1061-1065
[6]   A NOVEL BRAIN ATPASE WITH PROPERTIES EXPECTED FOR THE FAST AXONAL-TRANSPORT MOTOR [J].
BRADY, ST .
NATURE, 1985, 317 (6032) :73-75
[7]   INTRACELLULAR MOVEMENT OF FODRIN [J].
CHENEY, R ;
HIROKAWA, N ;
LEVINE, J ;
WILLARD, M .
CELL MOTILITY AND THE CYTOSKELETON, 1983, 3 (5-6) :649-655
[8]   Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT):: IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons [J].
Cole, DG ;
Diener, DR ;
Himelblau, AL ;
Beech, PL ;
Fuster, JC ;
Rosenbaum, JL .
JOURNAL OF CELL BIOLOGY, 1998, 141 (04) :993-1008
[9]   NOVEL HETEROTRIMERIC KINESIN-RELATED PROTEIN PURIFIED FROM SEA-URCHIN EGGS [J].
COLE, DG ;
CHINN, SW ;
WEDAMAN, KP ;
HALL, K ;
VUONG, T ;
SCHOLEY, JM .
NATURE, 1993, 366 (6452) :268-270
[10]   SYNAPSIN-I (PROTEIN-I), A NERVE TERMINAL-SPECIFIC PHOSPHOPROTEIN .2. ITS SPECIFIC ASSOCIATION WITH SYNAPTIC VESICLES DEMONSTRATED BY IMMUNOCYTOCHEMISTRY IN AGAROSE-EMBEDDED SYNAPTOSOMES [J].
DECAMILLI, P ;
HARRIS, SM ;
HUTTNER, WB ;
GREENGARD, P .
JOURNAL OF CELL BIOLOGY, 1983, 96 (05) :1355-1373