Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control

被引：367

作者：

Sosnowski, RG ^{[1
]}

Tu, E ^{[1
]}

Butler, WF ^{[1
]}

OConnell, JP ^{[1
]}

Heller, MJ ^{[1
]}

机构：

[1] NANOGEN INC,SAN DIEGO,CA 92121

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 04期

关键词：

D O I：

10.1073/pnas.94.4.1119

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have demonstrated that controlled electric fields can be used to regulate transport hybridization, and denaturation of single- and double-stranded oligonucleotides. Discrimination among oligonucleotide hybrids with widely varying binding strengths may be attained by simple adjustment of the electric field strength, When this approach Is used, electric field denaturation control allows single base pair mismatch discrimination to be carried out rapidly (<15 sec) and with high resolution, Electric field denaturation takes place at temperatures well below the melting point of the hybrids, and it may constitute a novel mechanism of DNA denaturation.

引用

页码：1119 / 1123

页数：5

共 11 条

[1] A NOVEL METHOD FOR NUCLEIC-ACID SEQUENCE DETERMINATION [J].