Synergic stimulation of laminin and epidermal growth factor facilitates the myoblast growth through promoting migration

被引:15
作者
Chowdhury, Shiplu Roy [2 ]
Muneyuki, Yuichi [1 ]
Takezawa, Yasunori [1 ]
Kino-oka, Masahiro [1 ]
Saito, Atsuhiro [3 ]
Sawa, Yoshiki [3 ]
Taya, Masahito [1 ,2 ]
机构
[1] Osaka Univ, Div Chem Engn, Grad Sch Engn Sci, Osaka 5608531, Japan
[2] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Dept Surg, Grad Sch Med, Suita, Osaka 5650871, Japan
关键词
Myoblast cells; Laminin; Epidermal growth factor; Migration; Cytokinesis; SKELETAL MYOBLASTS; MYOGENIC DIFFERENTIATION; KINASE ACTIVATION; HEART-FAILURE; CELLS; TRANSPLANTATION; INTEGRINS; MOTILITY; RECEPTOR;
D O I
10.1016/j.jbiosc.2009.03.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 090105 [作物生产系统与生态工程];
摘要
The dynamic behaviors of human skeletal muscle myoblasts were investigated in the culture on a laminin-coated surface in the presence of 100 ng/ml epidermal growth factor (EGF) in medium. The coexistence of laminin and EGF caused the enhancement of myoblast migration, giving an average migration rate of 62.0 mu m/h, which was 2.7 times that on a plain surface. This encouraged migration could be a driving force to separate the dividing cells from each other, accompanied by shortened disjunction time of daughter cells to complete cytokinesis. In addition, the synergic effect of laminin and EGF led to the promotion of myoblast growth with keeping a relatively high fraction of proliferative cells during the culture for 150 h, which is considered to arise from the reduced frequency of cell-cell contacts during cytokinesis and thereby suppressing the process towards myotube formation after cell division. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:174 / 177
页数:4
相关论文
共 20 条
[1]
Integrin Cytoplasmic domain-Associated Protein-1 (ICAP-1) promotes migration of myoblasts and affects focal adhesions [J].
Alvarez, Belen ;
Stroeken, Peter J. M. ;
Edel, Michael J. ;
Roos, Ed .
JOURNAL OF CELLULAR PHYSIOLOGY, 2008, 214 (02) :474-482
[2]
A natural hepatocyte growth factor scatter factor autocrine loop in myoblast cells and the effect of the constitutive met kinase activation on myogenic differentiation [J].
Anastasi, S ;
Giordano, S ;
Sthandier, O ;
Gambarotta, G ;
Maione, R ;
Comoglio, P ;
Amati, P .
JOURNAL OF CELL BIOLOGY, 1997, 137 (05) :1057-1068
[3]
Bischoff R, 1997, DEV DYNAM, V208, P505, DOI 10.1002/(SICI)1097-0177(199704)208:4<505::AID-AJA6>3.0.CO
[4]
2-M
[5]
Chachques JC, 2002, HERZ, V27, P570, DOI 10.1007/s00059-002-2422-3
[6]
Myoblast migration is regulated by calpain through its involvement in cell attachment and cytoskeletal organization [J].
Dedieu, S ;
Poussard, S ;
Mazères, G ;
Grise, F ;
Dargelos, E ;
Cottin, P ;
Brustis, JJ .
EXPERIMENTAL CELL RESEARCH, 2004, 292 (01) :187-200
[7]
EFFECT OF FIBROBLAST GROWTH-FACTOR ON DIVISION AND FUSION OF BOVINE MYOBLASTS [J].
GOSPODAROWICZ, D ;
WESEMAN, J ;
MORAN, JS ;
LINDSTROM, J .
JOURNAL OF CELL BIOLOGY, 1976, 70 (02) :395-405
[8]
Myoblast transplantation for cardiac repair: A clinical perspective [J].
Haider, HK ;
Tan, ACK ;
Aziz, S ;
Chachques, JC ;
Sim, EKW .
MOLECULAR THERAPY, 2004, 9 (01) :14-23
[9]
Netrins and neogenin promote myotube formation [J].
Kang, JS ;
Yi, MJ ;
Zhang, W ;
Feinleib, JL ;
Cole, F ;
Krauss, RS .
JOURNAL OF CELL BIOLOGY, 2004, 167 (03) :493-504
[10]
Evaluation of growth potential of human epithelial cells by motion analysis of pairwise rotation under glucose-limited condition [J].
Kino-Oka, M ;
Agatahama, Y ;
Hata, N ;
Taya, M .
BIOCHEMICAL ENGINEERING JOURNAL, 2004, 19 (02) :109-117