Linking phytochrome to plant immunity: low red : far-red ratios increase Arabidopsis susceptibility to Botrytis cinerea by reducing the biosynthesis of indolic glucosinolates and camalexin

被引:62
作者
Cargnel, Miriam D. [1 ]
Demkura, Patricia V. [1 ]
Ballare, Carlos L. [1 ,2 ]
机构
[1] Univ Buenos Aires, Consejo Nacl Invest Cient & Tecn, IFEVA, Buenos Aires, DF, Argentina
[2] Univ Nacl San Martin, Consejo Nacl Invest Cient & Tecn, IIB INTECH, Buenos Aires, DF, Argentina
关键词
auxin; Botrytis; camalexin; defense; glucosinolate; jasmonate; JAZ; tryptophan; TRANSCRIPTION FACTORS; SECONDARY METABOLITES; UV-B; SHADE-AVOIDANCE; JAZ REPRESSORS; NONHOST RESISTANCE; DEFENSE RESPONSES; SALICYLIC-ACID; CANOPY LIGHT; THALIANA;
D O I
10.1111/nph.13032
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Shade-intolerant plants respond to low red: far-red (R:FR) ratios, which signal the proximity of potential competitors, by down-regulating immune responses. Here we investigated the mechanisms underlying this immune suppression in Arabidopsis. We used genetic, transcriptomic and metabolomic approaches to examine the functional connections between R:FR ratio and Arabidopsis resistance to the fungus Botrytis cinerea. Low R:FR ratios reduced the concentration of indol-3-ylmethyl glucosinolate (I3M) (an indolic glucosinolate, iGS) and camalexin in plants inoculated with B.cinerea, and attenuated the I3M response triggered by jasmonate elicitation. These effects on metabolite abundance correlated with reduced expression of iGS and camalexin biosynthetic genes. Furthermore, the effect of low R:FR increasing Arabidopsis susceptibility to B.cinerea was not present in mutants deficient in the biosynthesis of camalexin (pad3) or metabolism of iGS (pen2). Finally, in a mutant deficient in the JASMONATE ZIM DOMAIN-10 (JAZ10) protein, which does not respond to low R:FR with increased susceptibility to B.cinerea, supplemental FR failed to down-regulate iGS production. These results indicate that suppression of Arabidopsis immunity against B.cinerea by low R:FR ratios is mediated by reduced levels of Trp-derived defenses, and provide further evidence for a functional role of JAZ10 in the link between phytochrome and jasmonate signaling.
引用
收藏
页码:342 / 354
页数:13
相关论文
共 88 条
[1]   Phytoalexins in defense against pathogens [J].
Ahuja, Ishita ;
Kissen, Ralph ;
Bones, Atle M. .
TRENDS IN PLANT SCIENCE, 2012, 17 (02) :73-90
[2]   PATHOGEN MORTALITY OF TROPICAL TREE SEEDLINGS - EXPERIMENTAL STUDIES OF THE EFFECTS OF DISPERSAL DISTANCE, SEEDLING DENSITY, AND LIGHT CONDITIONS [J].
AUGSPURGER, CK ;
KELLY, CK .
OECOLOGIA, 1984, 61 (02) :211-217
[3]   CYP83B1, a cytochrome P450 at the metabolic branch paint in auxin and indole glucosinolate biosynthesis in Arabidopsis [J].
Bak, S ;
Tax, FE ;
Feldmann, KA ;
Galbraith, DW ;
Feyereisen, R .
PLANT CELL, 2001, 13 (01) :101-111
[4]   Light Regulation of Plant Defense [J].
Ballare, Carlos L. .
ANNUAL REVIEW OF PLANT BIOLOGY, VOL 65, 2014, 65 :335-363
[5]   Canopy Light and Plant Health [J].
Ballare, Carlos L. ;
Mazza, Carlos A. ;
Austin, Amy T. ;
Pierik, Ronald .
PLANT PHYSIOLOGY, 2012, 160 (01) :145-155
[6]   Jasmonate-induced defenses: a tale of intelligence, collaborators and rascals [J].
Ballare, Carlos L. .
TRENDS IN PLANT SCIENCE, 2011, 16 (05) :249-257
[7]   Illuminated behaviour: phytochrome as a key regulator of light foraging and plant anti-herbivore defence [J].
Ballare, Carlos L. .
PLANT CELL AND ENVIRONMENT, 2009, 32 (06) :713-725
[8]   PHOTOCONTROL OF STEM ELONGATION IN PLANT NEIGHBORHOODS - EFFECTS OF PHOTON FLUENCE RATE UNDER NATURAL CONDITIONS OF RADIATION [J].
BALLARE, CL ;
SCOPEL, AL ;
SANCHEZ, RA .
PLANT CELL AND ENVIRONMENT, 1991, 14 (01) :57-65
[9]   MORPHOLOGICAL RESPONSES OF DATURA-FEROX L SEEDLINGS TO THE PRESENCE OF NEIGHBORS - THEIR RELATIONSHIPS WITH CANOPY MICROCLIMATE [J].
BALLARE, CL ;
SANCHEZ, RA ;
SCOPEL, AL ;
GHERSA, CM .
OECOLOGIA, 1988, 76 (02) :288-293
[10]   FAR-RED RADIATION REFLECTED FROM ADJACENT LEAVES - AN EARLY SIGNAL OF COMPETITION IN PLANT CANOPIES [J].
BALLARE, CL ;
SCOPEL, AL ;
SANCHEZ, RA .
SCIENCE, 1990, 247 (4940) :329-332