High-performance liquid chromatography-electrospray ionization-mass spectrometry study of ginkgolic acid in the leaves and fruits of the ginkgo tree (Ginkgo biloba)

被引：38

作者：

He, XG ^{[1
]}

Bernart, MW ^{[1
]}

Nolan, GS ^{[1
]}

Lin, LZ ^{[1
]}

Lindenmaier, MP ^{[1
]}

机构：

[1] AM Todd Bot, Res Lab Nat Prod Chem, Eugene, OR 97402 USA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2000年 / 38卷 / 04期

关键词：

D O I：

10.1093/chromsci/38.4.169

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is developed for qualitative analysis of ginkgolic acids in the leaves and fruits of Ginkgo biloba by high-performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry technique. Negative ionization mode is successful in obtaining a very abundant deprotonated molecule [M - H]-. The mass detection sensitivity is higher than ultraviolet detection but relies heavily on the concentration of acetic acid in the HPLC eluent, which consists of acetonitrile-water-acetic acid. The method is also very specific for the analysis of ginkgolic acid with no interferences from the sample matrix.

引用

页码：169 / 173

页数：5

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