A TSM immunosensor for detection of M-tuberculosis with a new membrane material

A novel thickness shear mode (TSM) immunosensor for detection of M. tuberculosis (M. TB) was presented in this paper. Styrene-butadiene-styrene block copolymer was selected as a new membrane material to immobilize antibody. The properties of the membrane, such as lifetime, response speed and the wettability, were studied. Using SBS copolymer-coated quartz crystal. the effective immobilization of antibodies was achieved on the TSM immunosensor surface, and a TSM immunosensor that based on the resonant frequency change was set up. A new method for diagnosis of M. TB was proposed based on this sensor. It was simple, unlabeled and accurate. when used in spike samples, good results were obtained. (C) 2002 Elsevier Science B.V. All rights reserved.