Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant

被引：27

作者：

Sakono, M

Maruyama, T

Kamiya, N

Goto, M ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8128581, Japan

[2] JST, PRESTO, Fukuoka 8128581, Japan

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2004年 / 19卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

protein refolding; carbonic anhydrase B; reversed micelle; nanobiotechnology;

D O I：

10.1016/j.bej.2004.01.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

We have succeeded in the efficient refolding of denatured carbonic anhydrase B (CAB) using reversed micelles formulated with nonionic surfactant. The reversed micelles with nonionic tetraethylene glycol dodecyl ether prevented denatured CAB from aggregation in the refolding process, allowing a refolding yield of over 70% in 20 h, while the refolding yield of CAB with AOT/isooctane reversed micelle was only about 5%. These results demonstrated that the reversed micelles-mediated protein refolding technique could be extended to the protein which has strong interaction with AOT by simply altering the type of surfactant. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：217 / 220

页数：4

共 13 条

[1]

Entrapment and condensation of DNA in neutral reverse micelles [J].