Persistent replication of the GBV-C subgenomic replicons in Huh7 cells

被引：3

作者：

Cao, Ming-Mei ^{[1
]}

Ren, Hao ^{[1
]}

Zhao, Ping ^{[1
]}

Pan, Wei ^{[1
]}

Chen, Qiu-Li ^{[1
]}

Qi, Zhong-Tian ^{[1
]}

机构：

[1] Second Mil Med Univ, Dept Microbiol, Shanghai 200433, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 157卷 / 02期

基金：

中国国家自然科学基金;

关键词：

GB virus C; Replicon; Replication; Viral vector; Gene therapy; Huh7; cells; HEPATITIS-G VIRUS; GENE-EXPRESSION; RNA REPLICATION; INFECTION; VARIANTS; SURVIVAL; CULTURE;

D O I：

10.1016/j.jviromet.2008.12.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Studies of GB virus type C (GBV-C) replication in vitro have been limited because of poor growth of GBV-C in cell culture. In order to address the infection of GBV-C, two GBV-C subgenomic replicons (GBCrepEGFP and GBCrepTNF) were developed from a GBV-C full-length genomic cDNA. The viral replication, protein expression and the production of virus-like particles were evaluated in human hepatoma cell line Huh7. The results showed that the established GBCrepEGFP and GBCrepTNF replicons could be replicated autonomously and expressed in cell culture for at least 2 months and I month respectively. The replicon RNA could assemble RNA-containing structures in the HuhEH cells expressing GBV-C structural proteins. It suggests that a cell line Supporting the replication of GBV-C was established. This replicon system might be used to understand better the biology of GBV-C. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：168 / 174

页数：7

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