The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity

被引：20

作者：

Flis, K

Bednarczyk, P

Hordejuk, R

Szewczyk, A

Berest, V

Dolowy, K

Edelman, A

Kurlandzka, A

机构：

[1] Polish Acad Sci, Dept Genet, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

[2] Agr Univ Warsaw, Dept Biophys, Chair Phys, SGGW, PL-02528 Warsaw, Poland

[3] Polish Acad Sci, Nencki Inst Expt Biol, Dept Cellular Biochem, PL-02093 Warsaw, Poland

[4] Fac Med Necker Enfants Malad, INSERM U467, F-75015 Paris, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 294卷 / 05期

关键词：

GEF1; chloride channel; CLC family; black lipid membrane (BLM); Saccharomyces cerevisiae;

D O I：

10.1016/S0006-291X(02)00610-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Gef1 protein of the yeast Saccharomyces cerevisiae (Gef1p) has amino acid homology to the voltage-gated CLC chloride channel family. It has been postulated that it provides the compensatory transport of Cl- anions to the lumen of the Golgi thereby regulating the pH of this compartment. Using GEF1 fusion with heterologous promoter we obtained a yeast strain highly overproducing Gef1p. The electrophysiological properties of the microsomal fraction obtained from this strain were measured using lipid bilayer system. Our data indicate that Gef1p is associated with the chloride channel activity. This anion-selective channel has a unitary conductance of 42 pS when measured in symmetrical 600/600 mM TEA-Cl solutions, is voltage-dependent, and closes at high negative voltages. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：1144 / 1150

页数：7

共 27 条

[1]

Ashcroft F.M., 2000, Ion Channels and Disease

[2] Multicopy suppressors of phenotypes resulting from the absence of yeast VDAC encode a VDAC-like protein [J].