Construction and evaluation of a porcine bacterial artificial chromosome library

被引：64

作者：

Suzuki, K

Asakawa, S

Iida, M

Shimanuki, S

Fujishima, N

Hiraiwa, H

Murakami, Y

Shimizu, N

Yasue, H ^{[1
]}

机构：

[1] Natl Inst Anim Ind, Dept Anim Breeding & Genet, Tsukuba, Ibaraki 3050901, Japan

[2] STAFF Inst, Res Div 2, Tsukuba, Ibaraki 3050854, Japan

[3] Keio Univ, Dept Biol Mol, Sch Med, Shinjyuku Ku, Tokyo 1600016, Japan

来源：

ANIMAL GENETICS | 2000年 / 31卷 / 01期

关键词：

swine; bacterial artificial chromosome; library;

D O I：

10.1046/j.1365-2052.2000.00588.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A porcine bacterial artificial chromosome (BAC) library consisting of 103 488 clones has been constructed. The average insert size in the BAC vector was calculated to be 133 kh, based on the examination of 189 randomly selected clones, indicating that the library contained 4.4 genome equivalents. The library can be screened by two-step PCR. The first screening step is performed on 22 superpools, each containing 4704 clones (49 x 96 well plates). In the second screening step, 49 plates comprising a superpool are arrayed in a 7 x 7 matrix and 4D-PCR is performed. Screening of the library superpools by PCR for 125 marker sequences selected from different regions of swine genome revealed 125 sequences, indicating that the library is not biased. Subsequent screenings (4D-PCR) were successfully applied for identification of clones containing each marker sequence. This porcine BAC library and the PCR screening system are useful for isolation of genomic DNA fragments containing desired sequences.

引用

页码：8 / 12

页数：5

共 15 条

[1] Construction and characterization of a large insert porcine YAC library [J].

Alexander, LJ ;

Smith, TPL ;

Beattie, CW ;

Broom, MF .

MAMMALIAN GENOME, 1997, 8 (01) :50-51

[2]

ANDERSON C, 1993, SCIENCE, V259, P1684, DOI 10.1126/science.8456291

[3] GENETIC-MAPPING OF QUANTITATIVE TRAIT LOCI FOR GROWTH AND FATNESS IN PIGS [J].

ANDERSSON, L ;

HALEY, CS ;

ELLEGREN, H ;

KNOTT, SA ;

JOHANSSON, M ;

ANDERSSON, K ;

ANDERSSONEKLUND, L ;

EDFORSLILJA, I ;

FREDHOLM, M ;

HANSSON, I ;

HAKANSSON, J ;

LUNDSTROM, K .

SCIENCE, 1994, 263 (5154) :1771-1774

[4]

[Anonymous], METHOD ENZYMOL

[5] THE PIGMAP CONSORTIUM LINKAGE MAP OF THE PIG (SUS SCROFA) [J].

ARCHIBALD, AL ;

HALEY, CS ;

BROWN, JF ;

COUPERWHITE, S ;

MCQUEEN, HA ;

NICHOLSON, D ;

COPPIETERS, W ;

VANDEWEGHE, A ;

STRATIL, A ;

WINTERO, AK ;

FREDHOLM, M ;

LARSEN, NJ ;

NIELSEN, VH ;

MILAN, D ;

WOLOSZYN, N ;

ROBIC, A ;

DALENS, M ;

RIQUET, J ;

GELLIN, J ;

CARITEZ, JC ;

BURGAUD, G ;

OLLIVIER, L ;

BIDANEL, JP ;

VAIMAN, M ;

RENARD, C ;

GELDERMANN, H ;

DAVOLI, R ;

RUYTER, D ;

VERSTEGE, EJM ;

GROENEN, MAM ;

DAVIES, W ;

HOYHEIM, B ;

KEISERUD, A ;

ANDERSSON, L ;

ELLEGREN, H ;

JOHANSSON, M ;

MARKLUND, L ;

MILLER, JR ;

DEAR, DVA ;

SIGNER, E ;

JEFFREYS, AJ ;

MORAN, C ;

LETISSIER, P ;

MULADNO ;

ROTHSCHILD, MF ;

TUGGLE, CK ;

VASKE, D ;

HELM, J ;

LIU, HC ;

RAHMAN, A .

MAMMALIAN GENOME, 1995, 6 (03) :157-175

[6] Human BAC library: Construction and rapid screening [J].

Asakawa, S ;

Abe, I ;

Kudoh, Y ;

Kishi, N ;

Wang, YM ;

Kubota, R ;

Kudoh, J ;

Kawasaki, K ;

Minoshima, S ;

Shimizu, N .

GENE, 1997, 191 (01) :69-79

[7] CONSTRUCTION AND CHARACTERIZATION OF A BOVINE BACTERIAL ARTIFICIAL CHROMOSOME LIBRARY [J].

CAI, L ;

TAYLOR, JF ;

WING, RA ;

GALLAGHER, DS ;

WOO, SS ;

DAVIS, SK .

GENOMICS, 1995, 29 (02) :413-425

[8]

Marklund L, 1996, ANIM GENET, V27, P255, DOI 10.1111/j.1365-2052.1996.tb00487.x

[9]

MIKAWA S, 1999, IN PRESS ANIMAL GENE

[10]

POMP D, 1995, J ANIM SCI, V73, P1408

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